Journal of the American College of Surgeons
Volume 210, Issue 3 , Pages 271-279 , March 2010

Low Oxygen-Affinity Hemoglobin Solution Increases Oxygenation of Partially Ischemic Tissue During Acute Anemia

  • Pedro Cabrales, PhD

      Affiliations

    • Corresponding Author InformationCorrespondence address: Pedro Cabrales, PhD, Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093

Received 3 September 2009 ,Revised 16 October 2009 ,Accepted 4 November 2009.

References 

  1. Sarani B, Strong M, Pascual J, Schwab CW. Necrotizing fasciitis: current concepts and review of the literature. J Am Coll Surg. 2009;208:279–288
  2. Nicholls TP, Shoemaker WC, Wo CC, et al. Survival, hemodynamics, and tissue oxygenation after head trauma. J Am Coll Surg. 2006;202:120–130
  3. Freeman BA, Crapo JD. Hyperoxia increases oxygen radical production in rat lungs and lung mitochondria. J Biol Chem. 1981;256:10986–10992
  4. Winslow RM. Blood substitutes. Curr Opin Hematol. 2002;9:146–151
  5. Alayash AI. Oxygen therapeutics: can we tame haemoglobin?. Nat Rev Drug Discov. 2004;3:152–159
  6. Winslow RM. Oxygen transport agents: a new approach to red blood cell alternatives. TATM. 2004;5:498–504
  7. Colantuoni A, Bertuglia S, Intaglietta M. Quantitation of rhythmic diameter changes in arterial microcirculation. Am J Physiol. 1984;246:H508–H517
  8. Endrich B, Asaishi K, Götz A, Messmer K. Technical report: a new chamber technique for microvascular studies in unanaesthetized hamsters. Res Exp Med. 1980;177:125–134
  9. Institute of Laboratory Animal Research, Commission on Life Sciences, National Research Council. Guide for the care and use of laboratory animals. Washington, DC: National Academy Press; 1996;
  10. Cabrales P, Tsai AG, Frangos JA, Intaglietta M. Role of endothelial nitric oxide in microvascular oxygen delivery and consumption. Free Radic Biol Med. 2005;39:1229–1237
  11. Altman DG, Bland JM. Statistics notes: how to randomize. BMJ. 1999;319:703–704
  12. Cabrales P, Tsai AG, Intaglietta M. Microvascular pressure and functional capillary density in extreme hemodilution with low and high plasma viscosity expanders. Am J Physiol. 2004;287:H363–H373
  13. Lipowsky HH, Zweifach BW. Application of the “two-slit” photometric technique to the measurement of microvascular volumetric flow rates. Microvasc Res. 1978;15:93–101
  14. Intaglietta M, Tompkins WR. Microvascular measurements by video image shearing and splitting. Microvasc Res. 1973;5:309–312
  15. Tsai AG, Friesenecker B, McCarthy M, et al. Plasma viscosity regulates capillary perfusion during extreme hemodilution in hamster skin fold model. Am J Physiol. 1998;275:H2170–H2180
  16. Kerger H, Groth G, Kalenka A, et al. pO2 measurements by phosphorescence quenching: characteristics and applications of an automated system. Microvasc Res. 2003;65:32–38
  17. Cabrales P, Tsai AG, Intaglietta M. Increased tissue PO2 and decreased O2 delivery and consumption after 80% exchange transfusion with polymerized hemoglobin. Am J Physiol. 2004;287:H2825–H2833
  18. Cabrales P, Tsai AG. Plasma viscosity regulates systemic and microvascular perfusion during acute extreme anemic conditions. Am J Physiol Heart Circ Physiol. 2006;291:H2445–H2452
  19. Tsai AG, Vandegriff KD, Intaglietta M, Winslow RM. Targeted O2 delivery by low-P50 hemoglobin: a new basis for O2 therapeutics. Am J Physiol Heart Circ Physiol. 2003;285:H1411–H1419
  20. D'Agnillo F, Alayash AI. Redox cycling of diaspirin crosslinked hemoglobin induces G2/M arrest and apoptosis in cultured endothelial cells. Blood. 2001;98:3315–3323
  21. Goldman DW, Breyer RJ, Yeh D, et al. Acellular hemoglobin-mediated oxidative stress toward endothelium: a role for ferryl iron. Am J Physiol. 1998;275:H1046–H1053
  22. Giulivi C, Cadenas E. Heme protein radicals: formation, fate, and biological consequences. Free Radic Biol Med. 1998;24:269–279
  23. Fitzpatrick CM, Savage SA, Kerby JD, et al. Resuscitation with a blood substitute causes vasoconstriction without nitric oxide scavenging in a model of arterial hemorrhage. J Am Coll Surg. 2004;199:693–701

 Disclosure Information: Nothing to disclose.

 This work was supported by Bioengineering Research Partnership grant R24-HL64395, Program project P01 HL071064, and grants R01-HL62354, R01-HL62318, and R01-HL76182.

PII: S1072-7515(09)01560-9

doi: 10.1016/j.jamcollsurg.2009.11.005

Journal of the American College of Surgeons
Volume 210, Issue 3 , Pages 271-279 , March 2010